Home > Browse Issues > Vol.37 No.9
Effects of Angelica sinensis Polysaccharide on Proliferation and Expression of Tumor-associated Fibroblasts Related Molecule of Bone Marrow Mesenchymal Stem Cells in the Lung Cancer Microenvironment
Wu Youming1, Zhang Qi1, Liu Yongqi1,2*, He Jianxin1, Wu Zhiwei1,2, Gao Zhuoyue1, Su Yun1, Luo Yali1, Zhang Liying1, Zhou Nina1
1Provincial-Level Key Laboratory for Molecular Medicine of Major Diseases and the Prevention and Treatment with Traditional Chinese Medicine Research in Gansu Colleges and Universities, Gansu University of Chinese Medicine, Lanzhou 730000, China;
2Key Laboratory of Dunhuang Medicine and Transformation at Provincial and Ministerial Level, Lanzhou 730000, China
2Key Laboratory of Dunhuang Medicine and Transformation at Provincial and Ministerial Level, Lanzhou 730000, China
Abstract: This work was aimed at investigating effects of Angelica sinensis polysaccharide (ASP) on proliferation and expression of TAFs related molecules of bone marrow mesenchymal stem cells (BMSCs) in the lung cancer microenvironment. The optimal concentration of ASP on the effect of Lewis lung cancer (LLC) and BMSCs was selected by CCK-8. After establishing the co-culture system of LLC and BMSCs by transwell chambers， we intervened it by the effective concentration of ASP. The study contained BMSCs， LLC， Co-BMSCs and ASP-Co-BMSCs group.The morphology of cells was observed by phase-contrast microscopy. The proliferation capability of cells was tested by CCK-8. The cell cycle was tested by flow cytometry. The protein expressions of biomarkers of TAFs-α-SMA and FAP of each group was detected by Western blot. Our results indicated that ASP (50 μg/mL) promoted BMSCs proliferation while had an obvious inhibitory effect on LLC proliferation (P<0.01). Compared with BMSCs group， the growth rate of Co-BMSCs increased significantly (P<0.01)， the ratio of cells in G0/G1 phase of Co-BMSCs group was reduced with an increase in S phase (P<0.05). Compared with Co-BMSCs group， the proportion of cells in G0/G1 phase of ASP-Co-BMSCs group was increased， and the proportion of cells in S phase was significantly decreased (P<0.05). In the results of Western blot， the protein expression of α-SMA and FAP in Co-BMSCs group raised obviously compared with that in BMSCs group (P<0.01). Compared with Co-BMSCs group， the expression of α-SMA and FAP protein decreased in the group of Co-BMSCs intervened with ASP (P<0.01). Our results suggested that ASP could inhibit the proliferation of BMSCs in lewis lung cancer microenvironment and expression of TAFs related molecule.
CN
EN