Research of Zedoary Turmeric Oil Induced Apoptosis of Human Gastric Carcinoma SGC-7901 Cells

Abstract: The study of this experiment was to explore the effects for the apoptosis trigged by zedoary turmeric oil (ZTO) on the human gastric carcinoma SGC-7901 cells. The effects of ZTO were studied by trypan blue staining method， light microscope， fluorescence microscope， transmission electron microscope， flow cytometry，Real-time PCR and Western blot. After the human gastric carcinoma SGC-7901 cells treated with various concentrations of ZTO for 48 h， the growth inhibition rate of cell was determined by trypan blue staining method.The morphology and ultrastructure were observed by light microscope， fluorescence microscope and transmission electron microscope. DNA fragmentation was assayed by agarose gel electrophoresis. The apoptotic rate was investigated by flow cytometry. The expression levels of Bax and Bcl-2 were explored by Real-time PCR and Western blot. The results showed that the concentration for the treatment was optimized to 110 μmol/L in this study，and the IC50 value was (108.002±0.305) μg/mL. The early apoptotic rate was reached to (25.07±0.82)% under the microscope. The typical DNA ladder was showed on agarose gel electrophoresis. Importantly， the expression level Bax increased and the expression level of Bcl-2 decreased， so that the ratio of Bax and Bcl-2 increased significantly (P<0.05). These results suggested that ZTO could induce the apoptosis of the human gastric carcinoma SGC-7901 cells by up-regulating the expression of Bax and down-regulating the expression of Bcl-2.

CSTR: 32200.14.cjcb.2015.09.0006