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A Comparative Histochemical Study on Mucous Cells in Digestive Tract of Eleutheronema tetradactylum with Juvenile， Young and Adult Fish
Xie Mujiao1,2, Ou Youjun1, Li Jiaer1*, Wen Jiufu1, Wang Pengfei1, Wang Wen1,2, Chen Shixi1,2
1Key Laboratory of South China Sea Fishery Resources Exploitation & Utilization, Ministry of Agriculture,South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences,Guangzhou 510300, China;
2College of Fisheries and Life Sciences, Shanghai Ocean University, Shanghai 201306, China
2College of Fisheries and Life Sciences, Shanghai Ocean University, Shanghai 201306, China
Abstract: Histochemical properties of mucous cells in digestive tract of Eleutheronema tetradactylum juvenile，young and adult fish were studied. The technology of AB-PAS (Alician blue and periodic acid Schiff reagent，Alician blue at pH2.5) staining was used in this paper. It displayed few type I mucous cells while many type II mucous cells in the esophagus of 35-day-old juvenile; Type I and type III mucous cells could be detected in foregut，the former seemed to be much in number than the latter. There were only type II mucous cells detected in midgut and hindgut， while no mucous cell was figured out in pyloric caeca. It could be described as a mounts of mucous cells exposing in the digestive tract of 65-day-old young fish. There were four types of mucous cells (I， II， III， IV) in esophagus and foregut. Density of mucous cells in esophagus had the highest value followed by hindgut， while mucous cells in other sections characterize had at least two types and rich in number. Though types of mucous cells are not rich as those in 65-day-old young fish， but the number of mucous cells was increasing extremely in adult fish. It showed mounts of type II and type IV mucous cells in esophagus， while mucous cells of pyloric caece were characterized as with the same types as esophagus， but not rich in number. Mounts of type I mucous cells exposed in mucosal epithelium of stomach and quiet a lot of type IV mucous cells were tested in gastric gland of stomach. Type III and type IV mucous cells in intestine could be distinguished as numerous， various in shape and big in size， and the density of mucous cells in foregut， midgut， and hindgut showed an increasing regularity. The result indicated that mucous cells development in E. tetradactylum of different development stages was improving from immature style to mature style.
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