Treadmill Exercise Promotes Nerve Vascular Regeneration in the Ischemia Penumbra by bFGF/Caveolin-1/VEGF Pathway

Abstract: This article investigated the effect of bFGF/Caveolin-1/VEGF signal pathway in treadmill training on promoting nerve vascular regeneration in the ischemia penumbra of rat brain. Rats were randomized into shamoperated group (S)， model group (M)， exercise model group (EM)， bFGF model group (bM)， bFGF and exercise model group (bEM). The protein levels of Caveolin-1， VEGF and bFGF in the ischemia penumbra of rats after MCAO were analyzed by Western blot. The positive cells number of CD34/VEGFR-2 and BrdU/Nestin were detected by double-staining immunofluorescence in the ischemia penumbra. The neurological behavior scores of each model group compared to S were all significant (P<0.05); There were no white infarcts in group S，while in other Model group there were visible different sizes of white infarcts by TTC; The number of VEGFR-2/CD34 and BrdU/Nestin double standard positive cells reached to peak at group bEM7d (P<0.05); Group EM bFGF intensity value was higher than the group M (P<0.05); Caveolin-1， VEGF intensity value reached to peak at group bEM7d (P<0.05). Lateral ventricle injection of bFGF could raise expression of Caveolin-1 and VEGF in the ischemia penumbra of rat brain. It is proved that the effect of treadmill exercise induced nerve vascular regeneration through bFGF/Caveolin-1/VEGF signaling pathway in the ischemia penumbra of rat brain.

CSTR: 32200.14.cjcb.2015.09.0004