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Analysis of Genes Involved in Safflower Flavonoids Biosynthesis Based on Safflower Transcriptome in Different Flowering
Liu Xiuming1,2, Zhang Yu2, Yao Na1, Cui Xiyan,Wang Liyong1, Dong Yuanyuan1, Wang Nan1, Li Xiaowei1,2, Li Haiyan1,2*, Li Xiaokun1,2*
1Ministry of Education Engineering Research Center of Bioreactor and Pharmaceutical Development, Jilin Agricultural University,Changchun 130118, China;
2College of Life Sciences, Jilin Agricultural University, Changchun 130118, China
2College of Life Sciences, Jilin Agricultural University, Changchun 130118, China
Abstract: 454 sequencing technology was applied to sequencing transcriptome of safflower samples of early flowering and full-bloom stage， respectively. We obained 577 664 and 562 930 Clean reads fragments， withaverage length 427 bp and 436 bp， respectively. 40 139 genes were expressed in early flowering in transcriptome.39 768 genes were expressed in full-bloom flowering through differential expression analysis. 28 316 genes were expressed simultaneously in these two samples. Massive annotation information related to metabolic was obtained through COG/KOG analysis. Among them， 24.681% was highest in annotating as basic functionality. Safflower flavonoids metabolic pathway was found and verified by Real-time PCR in KEGG Pathway analysis. Quantitative PCR analysis showed that the CHS had the highest expression level in the flowering period of two safflower var.
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