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Constructed CHO Cell Model to Detect the Activity of Glucagon-like Peptide-1 Analogues
Qian Kai1, Cai Yanfei2, Chen Yun2, Jin Jian2*
1School of Biotechnology, Jiangnan University, Wuxi 214122, China;
2School of Pharmaceutical Sciences, Jiangnan University, Wuxi 214122, China
2School of Pharmaceutical Sciences, Jiangnan University, Wuxi 214122, China
Abstract: The in vitro activity of glucagon-like peptide-1 (GLP-1) analogue was detected in cell which stimulated the glucagon-like peptide-1 receptor (GLP-1R). We constructed the cell model in Chinese hamster ovary (CHO) cells which could express hGLP-1R. Plasmid of pcDNA3.1/hGLP-1R was constructed， and then was transformed into CHO by electricity. Recombination CHO cell was selected， and confirmed by RT-PCR and flow cytometry. Results suggested that the cell model could not only transcript the gene of GLP-1R but also express the GLP-1R protein in the cell membrane. The cell model could well detect the bioactivity of GLP-1 analogue in vitro. We constructed a recombination CHO cell which could well be used to detect the bioactivity of GLP-1 analogue in vitro.
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