Home > Browse Issues > Vol.37 No.8
Current Progress in the Culture of Porcine Clone Embryos In Vitro
Zhang Xia1, Nie Zhengwen2, Miao Yiliang2*
1College of Veterinary Medicine, Huazhong Agricultural University,
Wuhan 430070, China;
2Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education,College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, China
2Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education,College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, China
Abstract: The culture of porcine clone embryos in vitro had always restricted the massive production of clone pigs. In the earlier time， the culture of porcine clone embryos used the culture system for embryos in vitro fertilization; however， it was not suitable for the development of porcine clone embryos. The main reason was that two different embryos had different metabolic ways and they had different periods on oxygen consumption. It was reported that oxygen consumption became increased from the compact morula stage for the embryos in vitro fertilization; nevertheless， it was increased after blastocyst stage for the clone embryos. So it would greatly improve the development of porcine clone embryos to establish a suitable culture system for embryo’s oxygen consumption. Furthermore， some additive agents could also improve the development of clone embryos. This paper reviewed the history and current situation on the study of culture of porcine clone embryos， the factors on culture (including of basic culture medium， various additive agents and oxygen pressure) and strategy for in vitro culture optimization， aiming to provide theoretical basis to establish best culture system to culture porcine clone embryos.
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