The Effect of Bone Mesenchymal Stem Cells Culture Medium on the Morphology， Growth and Differentiation of Neural Stem Cells

Abstract: This study was designed to observe the effect of BMSCs (bone mesenchymal stem cells) conditioned medium on the differentiation of NSCs (neural stem cells) after the isolation， culture and identification of SD rat BMSCs and NSCs. BMSCs were cultured using method of all-adherent culture and identified with specific antigen marker by flow cytometry. NSCs were cultured using serum-free method and identified with specific antigen marker by immunofluorescence. NSCs were induced with BMSCs conditioned medium (DMEM medium containing 10% fetal bovine serum) for 7 d， and then we could observe morphology of NSCs and detect NSCs differentiation using immunofluorescence technique. We found that BMSCs highly expressed CD90， CD29 (>90%)， whereas CD45 expression was rarely; and immunofluorescence showed that specific marker of NSCs such as Nestin， SOX2 was positive reaction. The positive rate of expression MAP-2 and GFAP were 73.80% and 50.47% respectively after NSCs were induced with BMSCs conditioned medium for 7 d. Meanwhile， after NSCs had been induced and differentiated by fetal bovine serum (FBS) for 7 d， immunofluorescence identification indicated that both MAP-2 and GFAP were positive as well， with the rates 42.14% and 31.90%， respectively. BMSCs conditioned medium can induce NSCs to differentiate into neurons and astrocytes， in which cytokines secreted by BMSCs maybe play the vital role.

CSTR: 32200.14.cjcb.2015.08.0007