Home > Browse Issues > Vol.37 No.7
Progress in ncRNAs Editing
Huang Lei, Wang Yajie, Chen Shi*
Key Laboratory of Combinatorial Biosynthesis and Drug Discovery, Ministry of Education, School of Pharmaceutical Sciences, Wuhan University, Wuhan 430071, China
Abstract: Noncoding RNAs (ncRNAs) have no protein-coding capability and they function directly in the form of RNA. Genes coding ncRNAs were called Junk DNA when they were discovered. However they are found to be related to increasingly number of diseases and become the focus of many researches. Loss-of-function models are indispensable for function digging， and there are different kinds of gene editing techniques such as gene targeting， zinc-finger nucleases (ZFN)， transcription activator-like effector nucleases (TALEN) and clustered regularly interspaced short palindromic repeat sequences/Cas9 (CRISPR/Cas9). However， the editing techniques used for ncRNAs are quite different from that of protein-coding genes. ncRNAs have no open reading frames so that small deletion may not disrupt the functions. In this review， research progress in miRNA and lncRNA are summarized as well as the techniques used to delete ncRNAs.
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