iTRAQ Quantitative Proteome Analysis of Metastasis-associated Proteins in Hepatocellular Carcinoma

Abstract: In this study， quantitative proteomics analysis was performed on hepatocellular carcinoma (HCC) cell lines with low and high metastatic potentials for discovery of candidate HCC metastasis-associated proteins.Two HCC cell lines (MHCC97H and HCCLM6)， which have the same genetic background but different metastatic potentials， were chosen for analysis. Proteins extracted from cells were analyzed by MS identification after FASP digestion and iTRAQ labeling. Bioinformatics analysis of differentially expressed proteins， including subcellular localization， biology process and molecular function enrichment， were performed using UniProt knowledgebase and GOfact software. A total of 5 033 proteins were identified and 5 013 of which were quantified. There were 91 differentially expressed proteins (|ratio|≥1.5， one sample t test， P<0.05)， among which 39 proteins were upregulated and 52 proteins were down-regulated in HCCLM6. Most of the differentially expressed proteins were located in cytoplasm and cell membrane. GO analysis suggested that proteins related to cell adhesion and protein binding were significantly enriched. This study profiled the proteomes of HCC cell lines with different metastatic potentials and discovered four novel candidate HCC metastasis-associated proteins (tubulin beta-2B chain， centromere protein F， laminin subunit alpha-5， vesicle-associated membrane protein 5)， which provided valuable reference for future studies on HCC metastasis.

CSTR: 32200.14.cjcb.2015.07.0011