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Effects of shRNA-mediated Knockdown of DEPDC7 on Proliferation，Migration and Invasion of Human Hepatoma Cell HepG2
Liao Zhijun1*, Wang Xinrui1, Yang Hong1, Zeng Yeting2
1Department of Biochemistry and Molecular Biology, Fujian Medical University, Fuzhou 350108, China;
2Department of Pathology and Institute of Oncology, School of Basic Medical Sciences, Fujian Medical University, Fuzhou 350108, China
2Department of Pathology and Institute of Oncology, School of Basic Medical Sciences, Fujian Medical University, Fuzhou 350108, China
Abstract: Function-unknown gene DEPDC7 (DEP domain containing 7) was obtained by gene chip data mining with highly selective expression in liver tissue. However， its role and molecular mechanism in hepatic cellular carcinoma are still unclear. In this study， we utilized liver cancer cell line HepG2 to construct DEPDC7 knockdown cell strain by RNAi. Moreover， we analyzed the mRNA and protein expression levels of DEPDC7 by RT-qPCR and Western blot. Cell proliferation and cell cycle were analyzed by methyl thiazol tetrazolium (MTT)， colony formation experiment and flow cytometry (FACS). Cell motility and invasiveness were assayed by Matrigel migration and invasion assay. The results showed that DEPDC7-shRNA effectively inhibited the expression of DEPDC7 at both mRNA and protein levels (P<0.05). Knockdown of DEPDC7 led to increased proliferation， migration and invasion (P<0.05). Our results suggested that shRNA-mediated knockdown of DEPDC7 significantly promoted cell proliferation，colony formation， migration and invasion. Moreover， this research pointed out the direction of future research on the mechanism of transcription and regulation of DEPDC7.
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