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Effects and Potential Mechanisms of Nanosecond Pulsed Electric Fields on Apoptosis Induction of Cisplatin-sensitive and Cisplatin-resistant Human Ovarian Cancer Cells
Zhang Shunquan, Yu Tinghe*
Department of Obstetrics and Gynecology, the Second Affiliated Hospital of Chongqing Medical University, Chongqing 400010, China
Abstract: Nanosecond pulsed electric fields (nsPEFs) have emerged as a promising anticancer strategy. This study explored growth inhibition， apoptosis induction and potential mechanisms of nanosecond pulsed electric fields in human ovarian cancer cells COC1 (cisplatin-sensitive cell line) and COC1/DDP (cisplatin-resistant cell line) in vitro. Cell viability was determined with CCK-8 assay， and the results demonstrated that nsPEFs could remarkably decrease cell viability of both the two cells in time dependent manners. Apoptosis was evaluated by Hoechst 33342 fluorescence staining and flow cytometry quantitatively， both the two assays revealed that nsPEFs could significantly induce apoptosis in COC1 and COC1/DDP cells. In addition， with JC-1 assay and luminescent assay respectively， we found mitochondrial membrane potential decreased and caspase-3 activity increased in COC1 and COC1/DDP cells after treating them with nsPEFs. Furthermore， we observed cell cycle by flow cytometry and found that nsPEFs induced cell cycle arrest in S phase in COC1 cells. Together， these data indicated that nanosecond pulsed electric fields could inhibit proliferation and induce apoptosis of COC1 and COC1/DDP cells， and the potential apoptosis pathway may be mitochondria mediated.
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