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The Responses of SENP3 to Different Extents of Oxidative Stress
Chu Huiling, Yan Qiongyu, Liu Kejia, Yi Jing, Yang Jie*
Department of Biochemistry and Molecular Cell Biology, Shanghai Key Laboratory of Tumor Microenvironment and Inflammation,Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China)
Abstract: In response to oxidation stress， proteins would alter their quantity， localization and activity to prevent from oxidative damage. Our previous study already discovered that SENP3 (Sentrin/SUMO specific protease 3) sensed certain extent of oxidative stress via inhibiting degradation and then giving rise to alteration of sumolylation of transcription factors， by which tumor obtained potential of proliferation and migration. However， how SENP3 senses and makes responses to distinct extents of oxidative stress are unclear. In the present study， we treated tumor cells with different doses of hydrogen peroxide (H2O2) to mimic different extent of oxidative stress and therefore detected the quantity and localization of SENP3 and further analyzed the changes of antioxidants expression affected by SENP3. The results showed that SENP3 exhibited quick accumulation under low level of H2O2 and other levels of H2O2 in a dose independent manner. Notably， SENP3 also translocated from nucleolus to nucleoplasm upon H2O2 in dose dependent manner. Moreover， SENP3 mediated upregulation of antioxidants， peroxiredoxin 4 (Prx4)， superoxide dismutase 1 (SOD1) and catalase (CAT) under different extents of oxidative stress. Taken together， SENP3 senses oxidative stress to be endowed with quantity regulation as well as localization regulation and then makes responses via gene expression regulation of specific antioxidants， indicating that SENP3 plays a critical role in elaborate redox maintenance and possesses physiology and pathology implications.
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