Influence of Caveolin-1 on VEGF Expression in OGD/R Human Umbilical Cord Blood Endothelial Progenitor Cells

Abstract: The purpose of this study was to provide experimental basis for the application of caveolin-1 in cerebral hypoxic-ischemic diseases through investigating the influence of caveolin-1 on VEGF expression in OGD/R (oxygen glucose deprivation/reoxygenation) human umbilical cord blood endothelial progenitor cells (EPCs). Density gradient centrifugation was adopted to obtain mononuclear cells from the cord blood， which were then inoculated to the culture plates coated with rat tail collagen-I and cultured using the endothelial cell nutrient solution of EGM-2. The proliferation and growth of EPCs were observed and identified through cellular morphology observation， double fluorescence staining， immunofluorescence cytochemistry staining， flow cytom etry， etc. The time points when EPCs were highly expressed were chosen to establish the caveolin-1-targeted RNA interference eukaryotic plasmids and build gene silencing and OGD/R models which were then randomly grouped into the control group， the OGD/R group， the caveolin-1 gene silencing group and the caveolin-1 gene silencing OGD/R group. Immunofluorescence and Western blot were adopted to detect the expression of caveolin-1 and VEGF. EPCs could be separated and obtained from human umbilical cord blood; caveolin-1 gene silencing models were successfully constructed; the expression of caveolin-1 and VEGF in the caveolin-1 gene silencing group was significantly lower than that in the control group (P<0.01)， but higher than that in the caveolin-1 gene silencing OGD/R group (P<0.05); and the expression of caveolin-1 and VEGF in the OGD/R group was significantly higher than that in the control group and caveolin-1 gene silencing OGD/R group (P<0.01). The results showed that caveolin-1 could affect the expression of VEGF of OGD/R EPCs， which should be one of favorable factors for vascular cytothesis.

CSTR: 32200.14.cjcb.2015.06.0015