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Hydroxysafflor Yellow A Inhibited Rat Vascular Smooth Muscle Cells Proliferation Induced by PDGF
Zhao Jingshan1,2*, Fang Mingxing3, Guo Qianshu4, Li Yunfeng1, Xu Bingyuan1, Lai Shaohong4, Feng Menghan1,4, Liu Yu1, Li Aiying1
1The Department of Biochemistry and Biology, Basic Medical College, Hebei University of Chinese Medicine, Shijiazhuang 050200, China; 2Hebei Key Laboratory of Chinese Medicine Research on Cardiocerebrovascular Disease, Shijiazhuang 050200, China; 3The Third Hospital of Hebei Medical University, Shijiazhuang 050052, China; 4Hebei Medical University, Shijiazhuang 050017, China
Abstract: Hydroxysafflor yellow A (HSYA) comes from safflowers that are well known as traditional Chinese medicine for the treatment of cardiovascular disease， and it belongs to flavonoids. Safflowers have the function of activating blood circulation to dissipate blood stasis. HYSA has been used to treat cardiovascular disease in clinic， but the treatment mechanism is unclear. The effect of hydroxysafflor yellow A on the proliferation of vascular smooth muscle cells (VSMCs) and the related mechanism were studied. The inhibitory effect of hydroxysafflor yellow A on VSMCs proliferation was detected using cell culture， MTT assay， Western blot and immunohistochemical staining. The results showed that HYSA inhibited cell proliferation induced by PDGF in a dose-dependent manner， reduced proliferating cell nuclear antigen (PCNA) expression and blocked PDGFR-MEK-ERK1/2 signaling pathway activated by PDGF in VSMCs. In conclusion， HYSA inhibited VSMCs proliferation via reducing the expression of PCNA and blocked signal transduction of MEK/ERK1/2 in VSMCs.
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