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Effect of TRBP2 Gene Over-expression on Proliferation， Migration and Invasion of Lung Adenocarcinoma Cell A549 and Its Mechanism
Xia Hongjun, Ge Mingjian*
Department of Cardiothoracic Surgery, the First Affiliated Hospital, Chongqing Medical University, Chongqing 400016, China
Abstract: In this study， we investigated the effect of TRBP2 (TAR RNA binding protein 2) gene over-expression on the proliferation， migration and invasion of lung adenocarcinoma A549 cells in vitro and explored the possible mechanism. The human lung adenocarcinoma A549 cells were infected with the lentivirus which recombinated the human TRBP2 gene in a proper MOI and cells stably over-expressing TRBP2 were constructed by observing fluorescence intensity. Then， the mRNA expression of TRBP2， MMP-2 and PKR were detected by fluorescence quantitative PCR (FQPCR) and the protein expression of TRBP2， MMP-2， PKR and p-PKR were detected by Western blot. MTT and colony formation assay were used to assess proliferation of A549 cells influenced by TRBP2 gene over-expression. Furthermore， Transwell chambers assay and adhesion experiments were used to detect the invasion， migration， homogeneous and heterogeneous cells intercellular adhesion among three groups. The results showed that A549 cells with the TRBP2 gene over-expressed were successfully built. Compared with the control and empty vector group， the over-expression TRBP2 gene cells’ invasion， migration ability and heterogeneous cells intercellular adhesion were enhanced (P<0.05)， while homogeneous cells intercellular adhesion were decreased (P<0.05). Abilities of proliferation and colony formation rate were enhanced (P<0.05). What’s more， the mRNA and protein expression of TRBP2 were significantly raised， so was the MMP-2 (P<0.05). The expression of p-PKR protein was significantly decreased， although the expression of PKR showed no difference. There was no any difference between the control group and empty vector group (P>0.05). In conclusion， by promoting the expression of MMP-2 and inhibiting the phosphorylation of PKR， over-expression of TRBP2 could promote the proliferation， invasion and migration of lung adenocarcinoma A549 cells.
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