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The Impact of Interfering YAP1 on the Biological Function of Eca-109 Cells
Song Jie1, Wu Qingchen1*, Zhang Cheng1, Wang Delin2
1Department of Cardiothoracic Surgery, the First Affliated Hospital, Chongqing Medical University, Chongqing 400016, China; 2Department of Urology, the First Affliated Hospital, Chongqing Medical University, Chongqing 400016, China
Abstract: To research the impact of interfering Yes-associated protein 1 (YAP1) on the biological function of Eca-109 cells， YAP1 shRNA was cloned into lentivirus vector. Then Eca-109 cell line was infected by the lentivirus vector. The mRNA level of YAP1 was detected by qPCR. The expressions of YAP1 and P53 in stable were analyzed by Western blot. The apoptosis of Eca-109 was estimated with FCM. And the cell proliferation of Eca-109 was analyzed by CCK-8 assay. The expression of YAP1 in transfected group was inhibited signifcantly， and the expression of p53 in transfected group was overexpressed signifcantly. The apoptosis of transfected group increased compared with the other groups. And the proliferation rate of transfected cells was lower than that of untransfected cells. The result demonstrated that interfering YAP1 could promote Eca-109 cells apoptosis and inhibit the cell proliferation， and its resistance to apoptosis might be partly related to the p53 gene.
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