Home > Browse Issues > Vol.37 No.6
Efficiency Improvement in Generation of Gene Targeted Mice with C57BL/6 Embryonic Stem Cells
Zhou Shuya1, Zuo Qin1, Liu Susu1, Wang Chenfei2, Li Baowen1, He Zhengming1, Fan Changfa1*
1Laboratory Animal Resource Center, Institute for Laboratory Animal Resources, National Institute of Food and Drug Control, Beijing 100050, China; 2Beijing Biocytogen Co., Ltd., Beijing 101111, China
Abstract: Pluripotency of a C57BL/6 embryonic stem (ES) cell line， which was named as B6-1-6， was identified in vitro and in vivo. To improve efficiency of generating gene targeted mice， 37 different gene targeted mice were generated with B6-1-6. Different ways of inducing BALB/c mice ovulation were compared， and the correlation between the apparent chimerism and germline transmission efficiency was analyzed. Based on our data， we found that natural ovulation with estrus induction was superior to hormone superovulation for BALB/c mice (2.91 blasto./mouse vs 0.82 blasto./mouse). For C57BL/6 ES cell line B6-1-6， 65~97 blastocysts should be injected to generate positive gene targeted mice with 95% success rate. In addition， we also found that the germline transmission efficiency was positively correlated with coat color chimerism level to a certain extent (r=0.316， P=0.057); and it was positively correlated with eye color chimerism level significantly (r=0.328， P<0.05). In this study， we explored a superior way to obtain BALB/c blastocysts and optimum number for microinjection. What’s more， the correlation between apparent chimerism level and germline transmission was discussed. Therefore， it will be helpful to improve the efficiency of generating gene targeted mice with C57BL/6 ES cells in future application.
CN
EN