Design and Exploration for Experimental Teaching on Endocytosis of Mammalian Cells

Abstract: Endocytosis plays a very important role in transport across membrane. However， traditional experiment teaching lacks the relevant content. To optimize the course system and content of cell biology experimental curriculum， equip students with advanced technologies and achieve the aim of cultivating creative talents， a pratical simplified method for experimental teaching of animal cell culture and endocytosis was designed. Professional phagocytes (murine peritoneal macrophages) and non-professional phagocytes (HeLa cell) were cultured in vitro. Pinocytosis was labelled with fluorescent marker dextran and was observed. Maorophage phagocytosis in vivo and in vitro was detected by means of chicken erythrocytes phagocytosis assay. Pinocytosis and phagocytosis could be displayed more integratedly and directly by this experimental teaching. The experimental contents enhanced students’ capability to analyze and solve problems in a comprehensive way， which laid a solid foundation for training highquality skill talents.

CSTR: 32200.14.cjcb.2015.05.0016