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A Screening for Functional microRNA in the Development of Drosophila Ovarian Follicle Cell Lineage
Zhao Kun, Li Mengjie*, Xin Tianchi, Xuan Tao, Li Mingfa
College of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China
Abstract: As negative regulators of gene expression， microRNA (miRNA) plays important roles during Drosophila development. In this paper， we aimed to investigate the effects of miRNA on the commitment of Drosophila ovarian follicle cell lineage， which is an in vivo genetically tractable model to understand the mechanisms of cell fate determination and cell migration. To identify functional miRNA in the process， we performed a phenotypic screening for total 31 Drosophila miRNA by UAS/GAL4 binary expression system. The results showed that several miRNA cause multiple severe defects during oogenesis. And both the overexpression and knock-down of miR-7 could block the border cell migration. miR-1， miR-124 and miR-263b may function on stalk cell induction， border cell migration and egg shell patterning. The results demonstrated that UAS/GAL4 system-based strategy used in this paper was feasible for functional miRNA identification.
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