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The Influence of DOK2 on the Biological Behavior of Gastric Cancer Cells and Its Relationship with the Prognosis of Gastric Cancer
Jiao Jinxia1, Wang Yongfei1*, Li Ming1, Yang Yang1, Zhang Ning2, Xing Xiangbin3, Ma Sanmei1*
1Department of Biotechnology, Jinan University, Guangzhou 510632, China; 2Physiology Institute, Guangzhou Medical University, Guangzhou 510182, China; 3Department of Gastroenterology, First Affiliated Hospital, Sun Yat-Sen University, Guangzhou 510080, China
Abstract: In this research， the recombinant plasmid pcDNA3.1-DOK2 was constructed and transfected into BGC823 cells. We evaluated the influence of over-expression of DOK2 on gastric cancer cells biological behavior by CCK8， soft agar and colony formation assay. The expression of DOK2 protein was detected by immunohistochemistry in 116 cases of gastric carcinoma， and its relationship with clinicopathological characteristics and prognosis of gastric cancer was analyzed. The results showed that over-expression of DOK2 significantly inhibited the growth， proliferation and colony of BGC823 cells. Immunohistochemistry showed the low expression of DOK2 protein in gastric cancer accounted for 62.93%， and DOK2 protein expression had a significant inverse correlation with depth of tumor invasion， lymph node metastasis and tumor differentiation degree; DOK2 protein expression， depth of tumor invasion， lymph node metastasis， distant metastasis and tumor differentiation degree were important factors affecting the prognosis of patients survival.
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