Differential Expression Analysis and Targets Identification of microRNA in Leiomyomas

Abstract: MicroRNA (miRNA) belongs to the small non-coding RNA family， which contributes broadly to most human physiological and pathological process. Recent report indicated that miRNAs were also involved in the pathogenesis of leiomyomas. In this study， we proposely to explore the differentially expressed miRNAs and identify their targets in leiomyomas. The results showed that three miRNAs (miR-363， miR-490 and miR-135b) were up-regulated for 4~6 folds， and three miRNAs (miR-217， miR-590 and miR-451) were down-regulated for 3~5 folds. We also analyzed the expression level of several predicted targets of the above miRNAs， and found that FNIP1 and SLC12A5 (miR-363 targets)， NR3C2 (miR-135b target)， ZNF367 and YOD1 (miR-590 targets) were significantly changed. Reporter assay revealed that the 3’UTRs of FNIP1， NR3C2 and ZNF367 could be bound directly by relative miRNA. Moreover， negative expression correlations were found between these miRNAs and their targets， which further demonstrated them as the direct functional targets. Our founding will supply new potentials for leiomyomas molecular mechanism study， diagnosis and therapy.

CSTR: 32200.14.cjcb.2015.05.0010