Home > Browse Issues > Vol.37 No.5
Establishment of iPSC Lines from A Polycythemia Vera Patient
Wang Wenjun, Xing Wen, Bai Jie, Liu Shuping, Xu Jing, Ji Guangzhen, Yuan Weiping, Zhang Xiaobing, Cheng Tao, Zhou Yuan*, Yang Fengchun
State Key Laboratory of Experimental Hematology, Institute of Hematology & Blood Diseases Hospital, Center for Stem Cell Medicine, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin 300020, China
Abstract: Induced pluripotent stem cell technology provides great advantages in basic medical research and clinical application. JAK2V617F is the predominant mutation in myeloproliferative neoplasms (MPN)， such as polycythemia vera (PV). In this study， we used episomal vectors to generate induced pluripotent stem cell (iPSC) from a PV patient carrying the JAK2V617F mutation. The result showed that the iPSC lines we established were stable and could be passaged repeatedly. The expression levels of pluripotent genes were similar to those of ES cells. In addition， these iPSCs carried different allele burdens of JAK2V617F mutation and were able to form teratomas in vivo. Modeling PV with these iPSC lines will provide a new approach to study the pathogenesis as well as to screen of the compounds targeting JAK2V617F for clinical therapy.
CN
EN