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A New Chemical Hypoxia Model of Pulmonary Artery Smooth Muscle Cells Induced by Sodium Sulfite
Bao Caiying1, Ye Yuzhu1, Zhu Chengwu1, Xue Binbin1, Yuan Peigen1, Jin Lida1, Wang Wantie2, Lin Lina1*
1The Department of Anesthesiology, The First Affiliated Hospital, Wenzhou Medical University, Wenzhou 325000, China; 2The Department of Physiolugy of Wenzhou Medical University, Wenzhou 325000, China
Abstract: Different concentrations of sodium sulfite of 1.5， 2.0， 3.0 g/L were used to make a new chemical hypoxia model of pulmonary artery smooth muscle cells (PASMCs). Hematoxylin-eosin (HE) staining and Cell Counting Kit-8 (CCK-8) were performed to observe the effect of sodium sulfite on the PASMCs. To define the anoxic extent of new hypoxia model， the changes of reactive oxygen species and the expression of HIF-1α were detected. The content of ROS tested by fluorescence microscope and the expressions of HIF-1α tested by RT-PCR and Western blot indicated that the levels of hypoxia were dependent on the concentration of the sodium sulfite. Although there were some damages to the PASMCs in the hypoxia model induced by 2.0 g/L and 3.0 g/L， but the model induced by 1.5 g/L of sodium sulfite was similar to the model induced by three gas incubator. So the concentration of sodium sulfite with 1.5 g/L was the best to induce a hypoxia model.
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