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iNOS Inhibitor 1400W Enhanced Chemotherapeutic Effect of Cisplatin by p53 Signal in SiHa Cervical Cancer Cells
Ye Jun1, Cheng Yan2, Chen Yaping1*
1Department of Gynaecology and Obstetrics, Shanghai 5th People’s Hospital of Fudan University, Shanghai 200240, China; 2Department of Gynaecology, the First People’s Hospital of Taicang, Taicang 215400, China
Abstract: The aim of this paper was to explore the effects of iNOS inhibitor 1400W combined with cisplatin to cervical cancer cell 1ine SiHa and the mechanisms. The effects of 1400W and cisplatin on SiHa cell growth were investigated by MTT. The SiHa cell apoptosis rate was detected by flow cytometry. The expressions of p53， MDM2 (murine double minut 2) and DNA-PK (DNA-dependent protein kinase) mRNA and protein in SiHa cell were detected by quantitative RT-PCR and Western blot. The results showed that 1400W combined with cisplatin could obviously inhibit SiHa cell growth， and showed a dose-dependent inhibitory effect on SiHa cell proliferation. 1400W enhanced the effect of DDP-induced cell apoptosis. Further study revealed that 1400W could inhibit DDP-induced overaccumulation of p53 and increased the expression of MDM2 and DNA-dependent protein kinase (DNA-PK). All of our data suggested that the combinational usage of iNOS inhibitor and DDP was an new strategy in cervical cancer treatment， which could improve the therapeutic effect of DDP on cervical cancer.
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