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2-Cyclopentylamino-5，8-Dimethoxy-1，4-Naphthoquinone Inhibited the LPS Induced ROS and NO Production in RAW264.7 Cells
Liu Lei1#, Shen Guinan1#, Feng Li1, Han Yinghao1, Luo Yinghua2, Jin Yinghua3, Jin Chenghao1, Cui Yudong1, Sun Hunan1*
1College of Life Science and Technology, Heilongjiang Bayi Agricultural University, Daqing 163319, China; 2College of Animal Science and Technology, Heilongjiang Bayi Agricultural University, Daqing 163319, China; 3Heilongjiang Bayi Agricultural University Library, Daqing 163319, China
Abstract: In inflammation processes， controlling the macrophage nitric oxide (NO) production and cellular reactive oxygen species (ROS) levels are the essential tools for inhibiting the LPS induced macrophage hyper-activation and macrophage mediated acute inflammation responses. In the present study， we examined the inhibitory effect of 5，8-dimethoxy-1，4-naphthoquinone (DMNQ) derivatives on LPS induced NO production and cellular ROS level in RAW264.7 macrophage cells. The results showed that among the six compounds， 2-Cyclopentylamino-5，8-dimethoxy-1，4-naphthoquinone significantly inhibited NO production and cellular ROS level in RAW264.7 cells， though it did not affect the cell phagocytosis. Furthermore， the signaling pathway studies showed that #6 compound significantly down-regulated the ROS dependent activation of ERK and JNK signaling pathway through inhibiting their phosphorylations， to decrease the NO production and inducible nitric oxide synthase (iNOS) protein expression.
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