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Effects of C/EBP β on APP Gene Expression
Chai Juan1, Li Yongling2, Lu Xiaohong2, Zhang Qian2, Ma Lin1, Huichen Wang3, Sun Tao1, Cui Jianqi1,2*
1Ningxia Key Laboratory of Cerebrocranial Diseases, the Incubation Base of National Key Laboratory; 2School of Basic Medical Sciences, Ningxia Medical University, Yinchuan 750004, China; 3Department of Radiative Oncology, Winship Cancer Institute, Emory University, Atlanta 30322, USA
Abstract: The C/EBP β lentivirus expression vector was successfully constructed and the viron was packaged by virus packaging kit in HEK293FT cells. Rat hippocampus neuron (HT22) was infected with the packaged viron for the study. The luciferase assay was performed to evaluate the effects of C/EBP β on APP promoter activities; the effects of C/EBP β on amyloid precursor protein (APP) and Sp1 gene expression were estimated with Q-PCR and Western blot. The results of luciferase assay demonstrated that C/EBP β could up-regulate APP promoter activities， and the results of Q-PCR and Western blot confirmed that C/EBP β could increase APP and Sp1 gene expressions both in transcriptional and translational levels. The possible mechanism for C/EBP β to upregulate APP gene expression may lie in C/EBP β facilitating the endogenous Sp1 gene expression， and the increasing expression of Sp1 directly promotes the APP gene expression.
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