Culture and Characterization of Intestine Epithelial Cells from Pheretima aspergillum (E. Perrier) In Vitro

Abstract: The intestine epithelial cell (IEC)， designated IEC-P.A.， was digested by complex enzyme from Pheretima aspergillum (E. Perrier)， which has been continuously subcultured 8 times in vitro. IEC-P.A. was identified by morphological and ultrastructure observation under inverted microscope and transmission electron microscope. The growth curve of IEC-P.A. was determined by MTT assay. Cell cycle and cell proliferation were observed by flow cytometry. IEC-P.A.， cultured in vitro， was about 8±2 μm in diameter， and appeared some distinct typical morphological characteristics of epithelial cells， such as cobblestone-like appearance， numerous microvilli， rough endoplasmic reticulum and mitochondria. Flow cytometric test result showed that more than 93% IEC-P.A. was in G0/G1 phase. Chromosome typing of IEC-P.A. revealed that the diploid chromosome number was 2n=22， and the chromosomal karyotype was n(♂)=x=6m+3sm+2st. IEC-P.A. was successfully cultured in vitro and its basic information on biological characteristics was investigated in this study.

CSTR: 32200.14.cjcb.2015.04.0006