MicroRNA-493 Inhibited Proliferation， Migration and Tumor Formation of Breast Cancer Cell MCF7

Abstract: To research the effect of miR-493 on cell proliferation， migration and tumor formation of breast cancer MCF7 cells， MCF7 cells were infected with recombinant plasmid pcDNA3.1(+)/miR-493 and cell lines with stably highly expressed miR-493 were selected by G418 screening. The expression of miR-493 in MCF10A， MCF7 cells and MCF7 clones after infection was detected by fluorescence quantitative Real-time PCR. In order to detect the influence of increased miR-493 expression in MCF7 cells， the proliferation ability was determined using CCK8 assay. The migration ability of MCF7 cells was assessed by Transwell and wound-healing assays. The invasion ability of MCF7 cells was detected by Transwell assay. The impact of miR-493 expression on the tumor formation ability of MCF7 cells was detected by soft agar assay in vitro and nude mice assay in vivo. The target gene was predicted by miRanda and TargetScan softwares and investigated by fluorescence quantitative Real-time PCR and Western blot. The result of CCK8 assay showed that over-expression of miR-493 could inhibit cell proliferation of MCF7 cells. The results of Transwell and wound-healing assays indicated that the abilities of migration and invasion of MCF7 cells were suppressed when miR-493 was up-regulated. And the results of soft agar and nude mice assays also revealed that over-expression of miR-493 could inhibit both clone formation and tumor formation of MCF7 cells.

CSTR: 32200.14.cjcb.2015.04.0005