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Subcellular Localization of Adenylate Cyclase III in Non-Neuronal Cells
Hao Jiangye1, Han Shaofang1, Luo Yingtao1, Yang Dong1, Li Chao1, Zhang Zhe1,2, Cao Zhenlong1, Hu Yuanxiang2, Liu Weili2, Ni Zhihua1, Zhou Yanfen1, Wang Zhenshan1*
1College of Life Sciences, Hebei University, Baoding 071002, China; 2College of Basic Medicine, Hebei University, Baoding 071002, China
Abstract: Adenylate cyclase III (AC3)， which was initially discovered in the main olfactory epithelium of mouse， is an important component of the cyclic adenosine monophosphate signaling pathway. Deletion of AC3 leads to olfactory loss， weight gain， male sterility， loss of maternal care behaviors， and declines in learning and memory abilities in mice. The majority of vertebrate cells have cilia， and AC3 is localized to the cilia of neurons in the mouse brain. Although many types of non-neuronal cells also express AC3， whether it is localized in the ciliary region of non-neuronal cells is yet to be determined. In this study， eight cell lines (two neuronal cells and six nonneuronal cells) were selected to investigate the localization of AC3 in non-neuronal cells. Semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR) and DNA sequencing indicated that AC3 was expressed in all of adthe test cells. Immunofluorescence staining showed that fluorescence signal of AC3 overlapped with that of cilia in the non-neuronal cell lines including IMCD3， 293T and bone marrow stromal cells. Additionally， AC3 was localized to the cilia of both A549 and HeLa cancer cells. These data indicate that AC3 is expressed in the ciliary region in non-neuronal cells.
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