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Mechano Growth Factor Promotes Proliferation and Migration of Human Cartilage Endplate Derived Stem Cells
Li Yuanjing, Liu Huan, Fan Xin, Zhou Yue*
Department of Orthopedics, Xinqiao Hospital, the Third Military Medical University, Chongqing 400037, China
Abstract: Mechano growth factor (MGF)， which expresses in a wide variety of tissues， can improve proliferation and migration of mechanocytes. To investigate the effects of MGF on proliferation and migration in human cartilage endplate derived stem cells (CESCs)， several assays including CCK-8， Transwell and Western blot were conducted. The results showed that MGF could improve CESCs proliferation and migration at a dose-dependent manner， and the most remarkable effects appeared at concentration of 4.5 ng/mL (P<0.05). When CESCs were pretreated with Erk inhibitor PD98059， the proliferation effect was decreased obviously (P<0.001). Pretreated with IGF-1R inhibitor PQ401， the effect on migration was highly reduced (P<0.001). Western blot analysis indicated that the phosphorylation level of Erk was higher after CESCs treated with MGF (P<0.001). Pretreated with IGF-1R inhibitor PQ401， the phosphorylation level of Erk was highly declined (P<0.001). These results confirm that MGF improves proliferation and migration of CESCs through the phosphorylation of Erk， and IGF-1R may play an important role in MGF promoting the migration of CESCs.
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