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Targeted Expression of Domain Regional Fragment of COX I Gene Alleviates Dysfunction of Mitochondria Induced by Sodium Azide
Xin Hu, Feng Tan1, Yin Xu, Yi Zheng, Jian-Xin Lu*
School of Medical Laboratory Science and School of Life Science, Wenzhou Medical College, Wenzhou 325035, China; 1The School of Basic Medical Sciences, Wenzhou Medical College, Wenzhou 325035, China
Abstract: To study the protective role of targeted expression of cytochrome c oxidase I (COX I) domain regional fragment in mitochondrial dysfunction and oxidative stress of SK-N-SH cells resulting from sodium azide (NaN3)， a mitochondrial targeting sequencing was inserted into domain regional fragment of COX I and the plasmid pEGFP-N1-COX I was constructed and transfected into the SK-N-SH cells. The studies showed that the expression of COX I markedly increased at the time of 24 h after transfection and persisted up to 72 h and directional expression of COX I domain regional fragment could not only maintain the mitochondrial potential but also inhibit the toxic effect on proliferation of cells induced NaN3. These data indicate that COX I domain regional fragment could prevent the cells from NaN3 toxicity， which may be related to the maintenance of mitochondrial function and inhibition of ROS production.
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