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The Study of Three-dimensional Neural Stem Cell Culture Models Using Various Hydrogel Scaffolds
Wu Yueting1,2,3, Li Zhaohui4*, Cui Zhanfeng4, Liu Qingxi3, Sun He3, Zhang Yaozhou5
1Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193,China; 2Tianjin International Joint Academy of Biotechnology and Medicine, Tianjin 300457, China; 3Tianjin Weikai Bioeng Ltd.,Tianjin 300457, China; 4The Institute of Biomedical Engineering, Oxford University, Oxford OX13PJ, U. K.; 5School of Pharmaceutical Science and Technology, Tianjin University, Tianjin 300072, China
Abstract: In this study， naturally derived hydrogels including collagen， hyaluronic acid (HA) and sodium alginate were selected as the main components and functionalized with laminin to produce 12 types of scaffolds.Applied with rat neural stem cells (rNSCs) cultured in vitro， the comparison of the biocompatibility and functions among these scaffolds was studied. The results showed that collagen and HA based scaffolds possessed threedimensional porous structures to support rNSCs adhesion after being cultured for 7 d. The 1 mg/mL of collagenbased scaffolds had superb support for rNSCs proliferation， while the synapses surrounded with the cells and even emerged the specific protein and shape of neuron in HA-based scaffolds functionalized laminin with 15% cross linker group. On the contrast， 1.5% sodium alginate-based scaffolds provided the microenvironment to allow neuronsphere formation， which were more suitable for large-scale dynamic culture of stem cell derived neurons. The selected hydrogels possessed excellent biocompatibility to support stem cell derived rNSCs viable and functioning during proliferation and differentiation. The in vitro 3D stem cell derived neuron model combined with various hydrogels developed here could be useful for neurodegenerative diseases research and drug testing.
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