Investigating the Migration and Invasion of Human Squamous Cervical Carcinoma Line Siha Stimulated by TGF-β1 and Its Underlying Mechanisms

Abstract: This research investigated the influence of transforming growth factor-beta1 (TGF-β1) on invasion and metastasis of human squamous cervical carcinoma cell line Siha， and might revealed some possible mechanisms.Cultured human cervical carcinoma cell line， Siha， was stimulated with 5 ng/mL of TGF-β1 for 72 h. The morphologic changes were observed under inverted microscope. Cell proliferation， clone-formation， adhering ability， migration and invasion ability changes were evaluated by CCK-8， colony-forming， cell adherence and Transwell assays， respectively. The expressions of matrix metalloproteinase-2 (MMP-2)， MMP-9， VEGF， CFTR， P50， P65， E-cadherin and Vimentin were detected by Western blot. We found that after TGF-β1 stimulation， Siha cells converted to an elongated spindle-like shape from a “cobblestone” epithelial structure. The migration and invasion ability of Siha cells were promoted dramatically. The expressions of MMP-2， MMP-9， VEGF， CFTR， P50， P65 and Vimentin were increased and E-cadherin was down-regulated than those of control groups. These results indicated that TGF-β1 might promote the migration and invasion of Siha cells through EMT and up-regulation of MMP-2， MMP-9 and VEGF.

CSTR: 32200.14.cjcb.2015.01.0009