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Identification and Functional Analysis of Rice Seedling Shoot Membrane Protein Regulated by Brassinosteroids
Gao Jing, Zhang Hao, Gao Mengzhu, Zhang Haili, Li Shenghui, Xing Jihong, Wang Fengru*, Dong Jingao*
Mycotoxin and Molecular Plant Pathology Laboratory, College of Life Science,Agricultural University of Hebei, Baoding 071001, China
Abstract: Brassinosteroids (BR) has obvious effect on important agronomic traits of rice， such as plant height and leaf angle. The identification of the proteins regulated by BR was important for revealing the potential mechanisms by which BR regulates specific biochemical and cellular processes in rice. In this study， Oryza sativa L. ssp. japanica cv.Nipponbare was used as research material， and the rice shoot was treated with BR which could induce obvious effect on rice shoot growth. Then we extracted the shoot membrane protein and analysed by two-dimensional electrophoresis and mass spectrometry. 7 proteins regulated by BR were identified: Photosystem II stability/assembly factor HCF136， PMRP (putative membrane related protein， gi|113565516)， ATP synthase (gi|113611230)， gi|113594641， gi|22831029， gi|47497322 and gi|56784135. PMRP was down regulated by BR. PMRP was mainly localized on cytoplasma membrane by subcellular localization analysis. Bioinformatics analysis showed that PMRP had a phosphatidylcholine binding site， so PMRP might influence the membrane components and play a role in the process of plant resistance. The PMRP RNAi transgenic Arabidopsis plants had enhanced resistance to chilling injury. These results prove that BR can improve the cold resistance of plants by regulating the expression of PMRP.
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