Extraction and Purification Groups from Eupolyphaga sinensis Walkers Inhibit Angiogenesis and Tumor Growth of the S180 Inoculated Mice

Abstract: This study was designed to evaluate the activities of anti-angiogenesis and anti-tumor with the protein extractions from Eupolyphaga sinensis， a kind of Chinese traditional medicine. The mice with S180 sarcoma inoculated were treated (i.g.) with the crude protein extractions at three different doses (0.2 g/ml， 0.4 g/ml， 0.8 g/ml， as crude materials)， then the inhibitory rate of tumor growth and some physical indexes of antioxidation of the mice were taken. The tumors peeled off the mice which were treated with the crude protein extractions were suppressed remarkably. Chick embryo chorioallantoic membrane (CAM) assay was used to determine the neovascularizative effect in vivo with the crude proteins extractions and the groups purified by ammonium sulphate precipitation， DEAE-cellulose and Sephadex G-75 Column chromatography， and the potent activities of anti-angiogenesis were exhibited respectively. All of our data suggest that the proteins extracted from Eupolyphaga sinensis with fibrinolytic activity must contain the potency to inhibit tumor growth and angiogenesis in vivo.

CSTR: 32200.14.cjcb.2007.03.0025