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Study on the Methods of In Situ Embedding Monolayer of a Few Cells for Electron Microscopy
Zhai Nan, Wu Yicheng, Chen Xinyu, Yi Jing, Yang Jie*
Department of Biochemistry and Molecular Cell Biology, Shanghai Jiaotong University School of Medicine, Shanghai 200025, China
Abstract: We proposed to investigate an optimized in situ embedding method of monolayer with a few cells for transmission electron microscopy. Cultured Hela cells were divided into 3 groups. Group 1: placed the resin capsules inversely on the plastic culture dishes during embedding preparation without propylene oxide. Group 2: placed the slides of cell samples inversely in the resin capsules during embedding preparation without propylene oxide. Group 3: placed the slides of cell samples inversely in the resin capsules during embedding preparation with propylene oxide. Cut the ultrathin sections of resin capsules which contained the cell samples from the 3 groups，and studied with transmission electron microscope. The results showed that the method used in Group 1 was the best among the three: the resin capsules did not contain the hole caused by missing of cell samples， and the ultrastructure was well kept. Moreover， embedding preparation steps were easy to operate.
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