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Study of Anti-tumor Immunity In Vitro Induced by DC Vaccines Loaded by p53-modified Tumor Antigens
Wang Bing1,2, Wang Hongmei1*, Liu Yanling1, Zhou Xiaoyan1, Zhou Huirong1, Xu Fangyun1, Min Weiping3
1Department of Pathophysiology, Medical College of Nanchang University, Nanchang 330006, China;
2Tongde Hospital of Zhejiang Province, Hangzhou 310012, China;
3Jiangxi Academy of Medical Sciences, Nanchang 330006, China
Abstract: Tumor antigen (p53-Ag) was extracted from mouse B16 melanoma cells transfected with the wild-p53 plasmid by freeze-thaw method. Then it was loaded onto dendritic cells (DC) harvested from the bone marrow to obtain DC tumor vaccine. The antigen-specific killing effect of cytotoxic T lymphocytes (CTL) activated by DC vaccine on cancer cells in vitro and the ability to induce proliferation of T cells were detected by MTT assay. The experiment results showed that p53-modified DC vaccine significantly up-regulated the proliferation of T lymphocytes and the killing rate by CTL on cancer cells.
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