Effects of Lipoxin A4 on Expression of G-CSF and Possible Mechanisms

Abstract: To explore the effects of lipoxin A₄ (LXA₄) on granulocyte colony stimulating factor (G-CSF) expression in RAW264.7 macrophages and the possible mechanisms. After treated with 1 mg/L lipopolysaccharide (LPS) in the absence or presence of LXA₄ (0.1-1000 nmol/L)， the cells were harvested and G-CSF gene expression levels were assessed by real time PCR， G-CSF protein concentrations were determined by ELISA， IκB_α degradation and NF-κB translocation were detected through Western blot， NF-κB transcriptional activities were tested by transfections and luciferase activities assay. Results demonstrated that LPS increased G-CSF gene and protein expression levels， LXA₄ inhibited the effects of LPS， and 10 nmol/L LXA₄ restrained LPS-induced IκB_α degradation， NF-κB translocation and NF-κB transcriptional activity. In a word， LXA₄ controlled LPS-induced G-CSF production through inhibition NF-κB activity in RAW264.7 macrophages.

CSTR: 32200.14.cjcb.2007.03.0021