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The Effects of Low-frequency Pulsed Electrical Field on the Synthesis of Tyrosine Hydroxylase and Dopamine in PC12 Cells
Yuan-Zhang Fang, Xiao-Jun Yu, Hong-Feng Zhang*, Shu-De Chen1
School of Life Science, East China Normal University; 1Key Laboratory of Ministry of Education for Optical and Magnetic Resonance Spectroscopy, Department of Physics, East China Normal University, Shanghai 200062, China
Abstract: The effects of low frequency pulsed electric field (LF-PEF) on the synthesis of tyrosine hydroxylase (TH) and dopamine (DA) were studied by Western blotting and HPLC. The results showed that short exposure to low-frequency pulsed electrical field (LF-PEF) (5， 10 min) could significantly activate the express of TH and DA after short culture period (2 days). The synthesis of TH and DA was inhibited in the longer exposed groups (15， 20， 30 min) within the same short culture period. After a long culture period， the synthesis of TH and DA was inhibited in the short exposed groups， while enhanced in longer exposed groups. The related signal pathways were tested by special inhibitor of protein kinase A (PKA) (H-89) and special inhibitor of mitogen activated protein-kinase kinase (MEK1/2) (U0126). The results suggested that PKA pathway was the most important pathway involved in the normal TH synthesis and the LF-PEF stimulated TH synthesis because H-89 could totally inhibit the synthesis of TH induced by LF-PEF.
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