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Differential Gene Expression Analysis of Chinese Kale during Regeneration by cDNA-AFLP and 2-D Electrophoresis
Ke Huang1,2,3, Xiao-Lin Yu1, Wan-Zhi Ye1, Xun Xiang1, Gang Lu1, Jia-Shu Cao1*
1Institute of Vegetable Sciences, Zhejiang University, Hangzhou 310029, China; 2Institute of Agricultural Product Quality, Fujian Agricultural and Forestry University, Fuzhou 350002, China; 3Institute of Vegetable Sciences
Abstract: The differential expression of Chinese kale during regeneration has been analyzed by cDNA-AFLP and 2-D electrophoresis. The results indicated that the differential gene expression of Chinese kale adventitious bud occurred at RNA level， the differential band appeared in explants with adventitious bud occurring mainly in 200-600 bp; and the differential gene expression also occurred at protein level， 160 differential proteins have been observed， among which 122 (76.25% of total differential proteins) proteins' pI were 5-7. Of the total differential proteins， 124 (77.5% of total differential proteins) proteins' MW distributed at 40-70 kDa.
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