Home > Browse Issues > Vol.29 No.1
The Establishment of Agrobacterium-mediated Transformation System in Brassica oleracea L. var. alboglabra
Ke Huang1,2,3, Wan-Zhi Ye1, Xiao-Lin Yu1, Xun Xiang1, Gang Lu1, Jia-Shu Cao1*
1Institute of Vegetable Sciences, Zhejiang University, Hangzhou 310029, China; 2Institute of Agricultural Product Quality, Fujian Agricultural and Forestry University, Fuzhou 350002, China; 3Institute of Vegetable Sciences
Abstract: By the orthogonalty design， the factors which influenced the transformation of Brassica oleracea L. var. alboglabra have been studied， the results showed that the major factor for KanR frequency is Kan concentration， and the major factor for Chinese kale transformation are pre-culture time and co-culture time. The best procedure for transformation is: Hypocotyl explants from 7-day-old germinated seedlings were pre-cultured for 2 d on pre-culture medium and co-cultivated for 2 d. The explants were then transferred to selection medium. Vigorous green shoot were transferred to rooted medium for 25 d when they grow at 2-3 cm. The vigorous seedlings were transplanted to vermiculite medium after exercised. In order to the results of PCR， Southern blot analyses， the gene CYP86MF have been transformated into the Chinese kale KanR plantlets.
CN
EN