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The Morphology and Membrane Potential of Primary Cultured Rat Hippocampal Neurons with Serum-free Media
Ming Zhang1, Yi Guo, Li Jiang*, Guan-Xin Liu
Department of Neurology, Children's Hospital, Chongqing University of Medical Sciences, Chongqing 400014, China
Abstract: Hippocampal neurons of neonatal Wistar rats were isolated and cultured in the serum-free B27 supplemented culture media. The process of hippocampal neurons growth was observed. Neurofilament (NF) was detected by immunofluorescence staining to identify neurons and to calculate the purity of neurons. Hippocampal neurons were labelled with voltage-sensitive fluorescent dye to monitor the effect of KCl on membrane potential of neurons by laser scanning confocal microscopy. In this study the fostered hippocampal neurons could survive over 20 days and develop completely mature from the 9th to 14th day in vitro. At the 7th day the purity of neurons reached 90%. And the depolarization of cells could be induced by KCl. It is suggested that the survival rate and purity of hippocampal neurons are high using this culture method. And the cultured hippocampal neurons have electrophysiological properties.
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