Resveratrol Triggers both Caspase Independent and Dependent Cell Death Pathway in T Lymphoblast Leukemia Jurkat Cells

Abstract: To study effects of resveratrol on acute lymphoblast leukemia Jurkat cell line， morphologic changes were observed under light microscope， fluorescence microscope and electron microscope. Cell death was determined by phosphatidylserine translocation and mitochondrial membrane permeabilization with flow cytometry (FCM). Western blot was conducted to detect the activation of caspase-3. DNA ladder was detected with gel electrophoresis. In resveratrol treatment groups， cell shrinkage， blebbing， margination and lumpy chromatin condensation， mitochondrial swelling and cristae membrane loss were observed under light microscope and electron microscope in treatment groups. More PI positive cells versus fewer annexin V positive cells were detected in 0.2 mmol/L and higher concentration groups at 24 h and 36 h check points in FCM evaluation. Western blot detected the activated 17 kDa caspase-3 in all treatment groups while negative in control. Cell death can be partly inhibited by pan-caspase inhibitor， Z-VAD-FMK. Gel electrophoresis displayed both DNA ladder and smear in resveratrol exposed groups， while DNA ladder disappeared in Z-VAD-FMK inhibiting group and left only smear. The above data suggest that resveratrol can induce both caspase dependent and independent cell death of Jurkat cells. Molecular understanding of resveratrol in Jurkat cell will be helpful in its future clinic application.
    

CSTR: 32200.14.cjcb.2007.01.0021