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Label-free and Dynamic Monitoring of Endothelial Barrier Function on Microelectronic Cell Sensor Arrays
Han-Bing Li1,2, Tong-Le Deng1, Qing-Nan Zhang1, Xiao-Xiang Zheng1*
1Department of Biomedical Engineering, Zhejiang University, Hangzhou 310027, China; 2College of Pharmaceutical Sciences, Zhejiang University of Technology, Hangzhou 310032, China
Abstract: The endothelium plays a vital role in the maintenance of vascular homeostasis and barrier dysfunction is a characteristic of many inflammatory conditions. In our study， an online and continuous technique based on real-time cell electronic sensing (RT-CES) was introduced into study of endothelial barrier function. Electrical impedance in human umbilical vein endothelial cells (HUVECs) was monitored in real-time on microelectronic cell sensor arrays using RT-CES from seeding to confluence. α-thrombin induced electrical impedance alteration in a dose-dependent manner which was paralleled by transendothelial FITC-dextran permeability across endothelial monolayer. Correspondingly， F-actin cytoskeleton redistribution of HUVECs accompanied the change of electrical impedance. The results suggest the RT-CES system could be a powerful tool to study endothelial barrier function model in vitro.
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