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Transport and Uptake of Chromium Chloride and Chromium Picolinate in Caco-2 Cell Monolayers
Long-Ying Zha, Zi-Rong Xu*, Min-Qi Wang, Liang-Ying Gu
Institute of Feed Science; The Key laboratory of Molecular Animal Nutrition, Ministry of Education, Zhejiang University, Hangzhou 310029, China
Abstract: In order to investigate the uptake and epithelial transport characteristics of chromium chloride and chromium picolinate in small intestine， a human intestinal epithelial cell model Caco-2 in vitro cultured was used to study the effects of time， concentration and temperature on uptake and transport of chromium chloride and chromium picolinate. The results showed that the uptake and transport of chromium chloride and chromium picolinate were increased linearly with the increase of concentration and incubation time， and were inclined to be decreased (P>0.05) when the incubation temperature was lowered from 37 ? to 4 ?. The apparent permeability coefficient (Papp) of chromium chloride and chromium picolinate from apical to basolateral was similar to that from basolateral to apical (0.95-1.41 fold and 0.84-1.07 fold respectively). The uptake rate of chromium chloride and chromium picolinate was (0.88?.08)% and (4.73?.60)%， respectively. The transport rate of chromium chloride and chromium picolinate was (2.11?.05)% and (9.08?.25)%， respectively. Thus， the uptake and transport of chromium chloride and chromium picolinate were passive diffusion as the dominating process.
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