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Extension of Yeast Two-hybrid System: Applications and Prospects of Reporter Protein Fragment Complementation and Functional Reconstitution Technology
Lin-Xia Song*
College of Life Science, Shandong University of Technology, Zibo 255049, China
Abstract: In this review， an improved two-hybrid system with technology of reporter protein fragment complementation and functional reconstitution is described. The principle is that reporter protein using in the system was dissected to two inactive fragments and could regain its function when the fragments fused to two interactive proteins respectively. This technology provided the extension of the traditional yeast two-hybrid system both in the detection methods and the range of cell types.
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