Home > Browse Issues > Vol.36 No.10
Efficient Genotyping ob Mice Using High Resolution Melting Analysis
Liu Susu, Zhou Shuya, Zuo Qin, Wang Chenfei, He Zhengming, Li Baowen, Liu Zuomin, Fan Changfa*
National Insitute for Food and Drug Control, Beijing 100059, China
Abstract: The ob/ob mouse is a well-characterized model of obesity in diabetes research， and market demands are increasing in recent years. For the C>T transition occurred in ob mouse， normal leptin protein cannot be produced. The ob homozygous mouse has similar exophenotype with wild type before 4 weeks old， and ob/ob mouse issterile and breeding of complete knockouts is impractical. Thus， fertile heterozygotes must be identified by genotyping methods to maintain efficient breeding colonies for the production of wild type， heterozygous and homozygous test animals. Here we described a new genotyping method based on high resolution melting analysis (HRM). The identification results by HRM are consistent with that of conventional PCR products gel-based method and DNA sequencing. The ob mice have expected exophenotype and blood glucose parameter when observed at 8 weeks old. This method is rapid， efficient and economical compared with other genotyping techniques.
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