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In Vitro Culture of Early Embryonic Mammary Anlagen Affects Expression Stabilities of Mammary Stem Cell-associated Markers
Song Jiazhe1, Du Liying2, Xue Kai1*
1College of Basic Medical Sciences, Dalian Medical University, Dalian 116044, China; 2College of Life Sciences, Peking University, Beijing 100871, China
Abstract: Mammary stem cells (MaSCs) possess the ability to generate all differentiated cell types in the mammary gland. The researches of MaSCs are important and fundamental for understanding mammary gland development， developing therapy of breast cancer and studying mammary gland bioreactor. In particularly， during embryonic development， fetal MaSCs undergo proliferation， migration and invasion， which do not occur in the resting adult mammary gland， and do resemble the progression of breast cancer. Therefore， the researches of fetal MaSCs are meaningful， especially in the field of medicine. Sca-1， CD29， CD49f and CD24 are specific cell surface markers that have ever been applied to purify MaSCs. Here， we analyzed the expression stabilities of these cell surface markers in mouse mammary anlagen， which were cultured in different conditions. Our research provides foundation to purify and identify MaSCs， and supports the studies of mammary gland development and breast cancer.
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