Home > Browse Issues > Vol.36 No.10
Inhibition of Cecropin XJ on Proliferation of Human Gastric Cancer AGS Cells
Wu Yanling, Xia Lijie, Zhang Fuchun*
Xinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology,Xinjiang University, Urumqi 830046, China
Abstract: Cecropin XJ， an antimicrobial peptide from Xinjiang silkworm， can inhibit tumor cells growth. To investigate the influence of different concentrations of Cecropin XJ on the growth of human gastric cancer AGS cells， several assays including MTT assay， soft agar colony formation assay， flow cytometry， wound-healing and Transwell assay were conducted. The results showed that 20~100 μg/mL of Cecropin XJ could significantly inhibit the AGS cells proliferation in a dose-dependent and time-dependent manner. After treated with 20， 50， 100 μg/mL of Cecropin XJ， colony forming ability was decreased by (35.81±13.10)%， (48.12±5.68)% and (81.46±6.21)%， respectively. The apoptosis rate of AGS cells increased gradually and the cell cycle was arrested in S phase after treated with different concentrations of Cecropin XJ for 24 h. 20 μg/mL of Cecropin XJ could decrease AGS cells migration and invasion， while 100 μg/mL of Cecropin XJ almost completely inhibited AGS cells migration and invasion. In conclusion， Cecropin XJ can significantly inhibit the growth and proliferation of AGS cells and might be potential for the treatment of human gastric cancer.
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